diff --git a/Mac/BuildScript/build-installer.py b/Mac/BuildScript/build-installer.py
index 16816d1cf86..241a9d1e508 100755
--- a/Mac/BuildScript/build-installer.py
+++ b/Mac/BuildScript/build-installer.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
 This script is used to build "official" universal installers on macOS.
 
@@ -1615,7 +1614,7 @@ def buildDMG():
     if os.path.exists(outdir):
         shutil.rmtree(outdir)
 
-    # We used to use the deployment target as the last characters of the 
+    # We used to use the deployment target as the last characters of the
     # installer file name. With the introduction of weaklinked installer
     # variants, we may have two variants with the same file name, i.e.
     # both ending in '10.9'.  To avoid this, we now use the major/minor
diff --git a/Modules/_sha3/cleanup.py b/Modules/_sha3/cleanup.py
index 4f53681b49e..73b263de94d 100755
--- a/Modules/_sha3/cleanup.py
+++ b/Modules/_sha3/cleanup.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+# Usage: ./python Modules/_sha3/cleanup.py
 # Copyright (C) 2012   Christian Heimes (christian@python.org)
 # Licensed to PSF under a Contributor Agreement.
 #
diff --git a/Objects/typeslots.py b/Objects/typeslots.py
index 8ab05f91be1..e9fd0b326ed 100755
--- a/Objects/typeslots.py
+++ b/Objects/typeslots.py
@@ -1,5 +1,4 @@
-#!/usr/bin/python
-# Usage: typeslots.py < Include/typeslots.h typeslots.inc
+# Usage: ./python typeslots.py < Include/typeslots.h typeslots.inc
 
 import sys, re
 
diff --git a/Parser/asdl_c.py b/Parser/asdl_c.py
index 34bd7010cfb..60629c169db 100755
--- a/Parser/asdl_c.py
+++ b/Parser/asdl_c.py
@@ -1,4 +1,3 @@
-#! /usr/bin/env python
 """Generate C code from an ASDL description."""
 
 import os
diff --git a/Python/makeopcodetargets.py b/Python/makeopcodetargets.py
index 023c9e6c9f1..bbdfc83d19c 100755
--- a/Python/makeopcodetargets.py
+++ b/Python/makeopcodetargets.py
@@ -1,4 +1,3 @@
-#! /usr/bin/env python
 """Generate C code for the jump table of the threaded code interpreter
 (for compilers supporting computed gotos or "labels-as-values", such as gcc).
 """
diff --git a/Tools/clinic/clinic.py b/Tools/clinic/clinic.py
index d4d77952468..de6b4994638 100755
--- a/Tools/clinic/clinic.py
+++ b/Tools/clinic/clinic.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3
-#
 # Argument Clinic
 # Copyright 2012-2013 by Larry Hastings.
 # Licensed to the PSF under a contributor agreement.
diff --git a/Tools/peg_generator/pegen/__main__.py b/Tools/peg_generator/pegen/__main__.py
index 1dcbaad1c38..55c2ddd856b 100755
--- a/Tools/peg_generator/pegen/__main__.py
+++ b/Tools/peg_generator/pegen/__main__.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3.8
-
 """pegen -- PEG Generator.
 
 Search the web for PEG Parsers for reference.